|
|
|
| Registros recuperados: 39 | |
|
|
| CALVO, E. S.. |
| Biotecnologia e o melhoramento genético de plantas. Mas o que é engenharia genética? Como assim? Como é o DNA? Mas como desenvolver novas variedade de plantas via engenharia genética? Como é feita a transformação? Transformação via agrobactéria e regeneração de plantas por organogênese. Como é feita regeneração de plantas num tubo de ensaio? Transformação com o revólver de genes. De que outra maneira a biotecnologia pode auliar no desenvolvimento de novas variedades? Regeneração de plantas de soja via embriogênese somática. Mas como os genes são identificados? O que é um organismo mutante? Como criar um mutante? Existem riscos associados com o uso de plantas transgênicas? |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Soja; Planta transgênica; Biotecnologia; Soybeans; Transgenic plants. |
| Ano: 1998 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/460384 |
| |
|
|
| Freitas-Astua,Juliana; Astua-Monge,Gustavo; Polston,Jane Elisabeth; Hiebert,Ernest. |
| Even though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still timeconsuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kanamycin; Genetically modified organisms; Transgenic plants; Testing. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700015 |
| |
|
|
| Torres,Antonio Carlos; Nascimento,Warley Marco; Paiva,Sonia Alessandra Vasconcelos; Aragão,Fernando Antonio Souza de. |
| Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 muM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Herbicide resistance; Transgenic plants; Biosynthesis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000900005 |
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa. |
| There is evidence that overexpression of transgenes codifying antifungal proteins may confer protection to pathogen attack, and that this protection is increased due to the synergic effect of the expression of two or more genes. On the other hand it is well known that sunflower is a recalcitrant specie, highly difficult to be genetically transformed. In this context, the final aim of this project was to obtain sunflower plants expressing at least two antifungal genes, avoiding sequential transformation. The antifungal genes used encode for two enzymes that degrade the fungal wall (glucanase and chitinase), an osmotin and a ribosome inhibitor protein. Two types of transformation vectors were used: a more traditional system with a double cassette and a novel... |
| Tipo: Journal article |
Palavras-chave: Antifungal genes; Double expression cassettes; Polyprotein cassettes; Sunflower; Transgenic plants. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500008 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Lee,Ki-Won; Choi,Gi Jun; Kim,Ki-Yong; Yoon,Sei Hyung; Ji,Hee Chung; Park,Hyung Soo; Lim,Young Chul; Lee,Sang-Hoon. |
| In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of seven cultivars were inoculated and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation effectiveness and the expression of the GUS gene were investigated. The highest transformation effectiveness (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium; Forage crop; Italian ryegrass; Transgenic plants. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300008 |
| |
|
| |
| Registros recuperados: 39 | |
|
|
|
